Expression of biologically active bovine interleukin 7 and evaluating the activity in vitro.

ABSTRACT:

Aim

Interleukin 7 (IL-7) is a ϒc family cytokine involved in the homeostatic proliferation and maintenance of immune cells. In the present study, we report the expression of bovine IL-7 (bIL-7) in Escherichia coli and evaluated for its biological activity.

Materials and methods

The sequence coding for bIL-7 (mature protein) was amplified from primary bovine kidney cell culture and cloned into pET28-a vector and expressed in E. coli (BL 21 DE3). The expressed protein was purified by nickel-nitrilotriacetatechromatography, and the reactivity of the protein was confirmed by western blotting using monoclonal antibodies raised against human IL-7. The biological activity of expressed bIL-7 was evaluated by analyzing its effect on the expression of anuclear factor for activated T-c

SUBMITTER: Lijo J 

PROVIDER: S-EPMC4819366 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature