Micro-proteomics with iterative data analysis: Proteome analysis in C. elegans at the single worm level.

ABSTRACT: Proteomics studies typically analyze proteins at a population level, using extracts prepared from tens of thousands to millions of cells. The resulting measurements correspond to average values across the cell population and can mask considerable variation in protein expression and function between individual cells or organisms. Here, we report the development of micro-proteomics for the analysis of Caenorhabditis elegans, a eukaryote composed of 959 somatic cells and ∼1500 germ cells, measuring the worm proteome at a single organism level to a depth of ∼3000 proteins. This includes detection of proteins across a wide dynamic range of expression levels (>6 orders of magnitude), including many chromatin-associated factors involved in chromosome structure and gene regulation. We apply the mi

SUBMITTER: Bensaddek D 

PROVIDER: S-EPMC4819713 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature