Assessing the impact of long term frozen storage of faecal samples on protein concentration and protease activity.

ABSTRACT:

Background

The proteome is the second axis of the microbiome:host interactome and proteases are a significant aspect in this interaction. They interact with a large variety of host proteins and structures and in many situations are implicated in pathogenesis. Furthermore faecal samples are commonly collected and stored frozen so they can be analysed at a later date. So we were interested to know whether long term storage affected the integrity of proteases and total protein and whether historical native faecal samples were still a viable option for answering research questions around the functional proteome.

Methods

Faecal samples were collected from 3 healthy volunteers (3 biological replicates) and processed in order to be stored at both -20 °C and -80 °C and in a variety

SUBMITTER: Morris LS 

PROVIDER: S-EPMC4819717 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature