Comprehensive translational control of tyrosine kinase expression by upstream open reading frames.

ABSTRACT: Post-transcriptional control has emerged as a major regulatory event in gene expression and often occurs at the level of translation initiation. Although overexpression or constitutive activation of tyrosine kinases (TKs) through gene amplification, translocation or mutation are well-characterized oncogenic events, current knowledge about translational mechanisms of TK activation is scarce. Here, we report the presence of translational cis-regulatory upstream open reading frames (uORFs) in the majority of transcript leader sequences of human TK mRNAs. Genetic ablation of uORF initiation codons in TK transcripts resulted in enhanced translation of the associated downstream main protein-coding sequences (CDSs) in all cases studied. Similarly, experimental removal of uORF start codons in addi

SUBMITTER: Wethmar K 

PROVIDER: S-EPMC4820681 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature