Proteogenomic Analysis of Mycobacterium smegmatis Using High Resolution Mass Spectrometry.

ABSTRACT: Biochemical evidence is vital for accurate genome annotation. The integration of experimental data collected at the proteome level using high resolution mass spectrometry allows for improvements in genome annotation by providing evidence for novel gene models, while validating or modifying others. Here, we report the results of a proteogenomic analysis of a reference strain of Mycobacterium smegmatis (mc(2)155), a fast growing model organism for the pathogenic Mycobacterium tuberculosis-the causative agent for Tuberculosis. By integrating high throughput LC/MS/MS proteomic data with genomic six frame translation and ab initio gene prediction databases, a total of 2887 ORFs were identified, including 2810 ORFs annotated to a Reference protein, and 63 ORFs not previously annotated to a Refer

SUBMITTER: Potgieter MG 

PROVIDER: S-EPMC4821088 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature