Production of biologically active IL-36 family cytokines through insertion of N-terminal caspase cleavage motifs.

ABSTRACT: Recent evidence has strongly implicated IL-36 cytokines as key initiators of inflammation in the skin barrier. IL-36 cytokines belong to the extended IL-1 family and, similar to most members of this family, are expressed as inactive precursors that require proteolytic processing for activation. Because the proteases responsible for activation of members of the IL-36 subfamily have not been reported, we have developed a method for the production of biologically active IL-36 through introduction of a caspase cleavage motif, DEVD, within the N-termini of these cytokines. Here, we show that DEVD-modified IL-36α, IL-36β and IL-36γ cytokines were highly soluble and were readily processed and activated by caspase-3. Caspase-3-processed IL-36 family cytokines exhibited robust biological activity o

SUBMITTER: Clancy DM 

PROVIDER: S-EPMC4821354 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature