Chemokines as novel and versatile reagents for flow cytometry and cell sorting.

ABSTRACT: Cell therapy regimens are frequently compromised by low-efficiency cell homing to therapeutic niches. Improvements in this regard would enhance effectiveness of clinically applicable cell therapy. The major regulators of tissue-specific cellular migration are chemokines, and therefore selection of therapeutic cellular populations for appropriate chemokine receptor expression would enhance tissue-homing competence. A number of practical considerations preclude the use of Abs in this context, and alternative approaches are required. In this study, we demonstrate that appropriately labeled chemokines are at least as effective in detecting their cognate receptors as commercially available Abs. We also demonstrate the utility of biotinylated chemokines as cell-sorting reagents. Specifically, we

SUBMITTER: Le Brocq ML 

PROVIDER: S-EPMC4821367 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature