Transcriptome Remodeling in Trypanosoma cruzi and Human Cells during Intracellular Infection.

ABSTRACT: Intracellular colonization and persistent infection by the kinetoplastid protozoan parasite, Trypanosoma cruzi, underlie the pathogenesis of human Chagas disease. To obtain global insights into the T. cruzi infective process, transcriptome dynamics were simultaneously captured in the parasite and host cells in an infection time course of human fibroblasts. Extensive remodeling of the T. cruzi transcriptome was observed during the early establishment of intracellular infection, coincident with a major developmental transition in the parasite. Contrasting this early response, few additional changes in steady state mRNA levels were detected once mature T. cruzi amastigotes were formed. Our findings suggest that transcriptome remodeling is required to establish a modified template to guide dev

SUBMITTER: Li Y 

PROVIDER: S-EPMC4821583 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature