Microfluidic droplet-based PCR instrumentation for high-throughput gene expression profiling and biomarker discovery.

ABSTRACT: PCR is a common and often indispensable technique used in medical and biological research labs for a variety of applications. Real-time quantitative PCR (RT-qPCR) has become a definitive technique for quantitating differences in gene expression levels between samples. Yet, in spite of this importance, reliable methods to quantitate nucleic acid amounts in a higher throughput remain elusive. In the following paper, a unique design to quantify gene expression levels at the nanoscale in a continuous flow system is presented. Fully automated, high-throughput, low volume amplification of deoxynucleotides (DNA) in a droplet based microfluidic system is described. Unlike some conventional qPCR instrumentation that use integrated fluidic circuits or plate arrays, the instrument performs qPCR in a

SUBMITTER: Hayes CJ 

PROVIDER: S-EPMC4822205 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature