Selection of reference genes for expression analysis in the entomophthoralean fungus Pandora neoaphidis.

ABSTRACT: The selection of suitable reference genes is crucial for accurate quantification of gene expression and can add to our understanding of host-pathogen interactions. To identify suitable reference genes in Pandora neoaphidis, an obligate aphid pathogenic fungus, the expression of three traditional candidate genes including 18S rRNA(18S), 28S rRNA(28S) and elongation factor 1 alpha-like protein (EF1), were measured by quantitative polymerase chain reaction at different developmental stages (conidia, conidia with germ tubes, short hyphae and elongated hyphae), and under different nutritional conditions. We calculated the expression stability of candidate reference genes using four algorithms including geNorm, NormFinder, BestKeeper and Delta Ct. The analysis results revealed that the comprehen

SUBMITTER: Chen C 

PROVIDER: S-EPMC4822748 | biostudies-literature | 2016 Jan-Mar

REPOSITORIES: biostudies-literature