Novel nonsense mutation in the katA gene of a catalase-negative Staphylococcus aureus strain.

ABSTRACT: We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme to just 283 amino acids.

SUBMITTER: Lagos J 

PROVIDER: S-EPMC4822749 | biostudies-literature | 2016 Jan-Mar

REPOSITORIES: biostudies-literature