A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics.

ABSTRACT: Awareness of the problem of antimicrobial resistance (AMR) has escalated and drug-resistant infections are named among the most urgent problems facing clinicians today. Our experiments here identify a transporter interactome and portray its essential function in acquisition of antimicrobial resistance. By exposing E. coli cells to consecutive increasing concentrations of the fluoroquinolone norfloxacin we generated in the laboratory highly resistant strains that carry multiple mutations, most of them identical to those identified in clinical isolates. With this experimental paradigm, we show that the MDTs function in a coordinated mode to provide an essential first-line defense mechanism, preventing the drug reaching lethal concentrations, until a number of stable efficient alterations occ

SUBMITTER: Shuster Y 

PROVIDER: S-EPMC4822809 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature