Chicoric acid binds to two sites and decreases the activity of the YopH bacterial virulence factor.

ABSTRACT: Chicoric acid (CA) is a phenolic compound present in dietary supplements with a large spectrum of biological properties reported ranging from antioxidant, to antiviral, to immunostimulatory properties. Due to the fact that chicoric acid promotes phagocytic activity and was reported as an allosteric inhibitor of the PTP1B phosphatase, we examined the effect of CA on YopH phosphatase from pathogenic bacteria, which block phagocytic processes of a host cell. We performed computational studies of chicoric acid binding to YopH as well as validation experiments with recombinant enzymes. In addition, we performed similar studies for caffeic and chlorogenic acids to compare the results. Docking experiments demonstrated that, from the tested compounds, only CA binds to both catalytic and secondary

SUBMITTER: Kuban-Jankowska A 

PROVIDER: S-EPMC4823031 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature