Polo-like kinase 1 mediates BRCA1 phosphorylation and recruitment at DNA double-strand breaks.

ABSTRACT: Accurate repair of DNA double-strand breaks (DSB) caused during DNA replication and by exogenous stresses is critical for the maintenance of genomic integrity. There is growing evidence that the Polo-like kinase 1 (Plk1) that plays a number of pivotal roles in cell proliferation can directly participate in regulation of DSB repair. In this study, we show that Plk1 regulates BRCA1, a key mediator protein required to efficiently repair DSB through homologous recombination (HR). Following induction of DSB, BRCA1 concentrates in distinctive large nuclear foci at damage sites where multiple DNA repair factors accumulate. First, we found that inhibition of Plk1 shortly before DNA damage sensitizes cells to ionizing radiation and reduces DSB repair by HR. Second, we provide evidence that BRCA1 fo

SUBMITTER: Chabalier-Taste C 

PROVIDER: S-EPMC4823034 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature