Concentration Dependent Ion-Protein Interaction Patterns Underlying Protein Oligomerization Behaviours.

ABSTRACT: Salts and proteins comprise two of the basic molecular components of biological materials. Kosmotropic/chaotropic co-solvation and matching ion water affinities explain basic ionic effects on protein aggregation observed in simple solutions. However, it is unclear how these theories apply to proteins in complex biological environments and what the underlying ionic binding patterns are. Using the positive ion Ca(2+) and the negatively charged membrane protein SNAP25, we studied ion effects on protein oligomerization in solution, in native membranes and in molecular dynamics (MD) simulations. We find that concentration-dependent ion-induced protein oligomerization is a fundamental chemico-physical principle applying not only to soluble but also to membrane-anchored proteins in their native e

SUBMITTER: Batoulis H 

PROVIDER: S-EPMC4823792 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature