Differential DNA methylation patterns of homeobox genes in proximal and distal colon epithelial cells.

ABSTRACT: Region and cell-type specific differences in the molecular make up of colon epithelial cells have been reported. Those differences may underlie the region-specific characteristics of common colon epithelial diseases such as colorectal cancer and inflammatory bowel disease. DNA methylation is a cell-type specific epigenetic mark, essential for transcriptional regulation, silencing of repetitive DNA and genomic imprinting. Little is known about any region-specific variations in methylation patterns in human colon epithelial cells. Using purified epithelial cells and whole biopsies (n= 19) from human subjects, we generated epigenome-wide DNA methylation data (using the HELP-tagging assay), comparing the methylation signatures of the proximal and distal colon. We identified a total of 125 diff

SUBMITTER: Barnicle A 

PROVIDER: S-EPMC4824152 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature