Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs.
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ABSTRACT:
SUBMITTER: Qu H
PROVIDER: S-EPMC4824429 | biostudies-literature | 2016
REPOSITORIES: biostudies-literature
Publications
Rapid and Inexpensive Method of Loading Fluorescent Dye into Pollen Tubes and Root Hairs.
Qu Haiyong H Xing Wenxi W Wu Fenfen F Wang Yongzhang Y
PloS one 20160407 4
The most direct technique for studying calcium, which is an essential element for pollen tube growth, is Ca2+ imaging. Because membranes are relatively impermeable, the loading of fluorescent Ca2+ probes into plant cells is a challenging task. Thus, we have developed a new method of loading fluo-4 acetoxymethyl ester into cells that uses a cell lysis solution to improve the introduction of this fluorescent dye into pollen tubes. Using this method, the loading times were reduced to 15 min. Furthe ...[more]
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