Characterization of Calflagin, a Flagellar Calcium-Binding Protein from Trypanosoma congolense.

ABSTRACT:

Background

Identification of species-specific trypanosome molecules is important for laboratory- and field-based research into epidemiology and disease diagnosis. Although Trypanosoma congolense is the most important trypanosome pathogen of cattle in Africa, no species-specific molecules found in infective bloodstream forms (BSF) of the parasites have been identified, thus limiting development of diagnostic tests.

Methods

Immuno-mass spectrometric methods were used to identify a protein that is recognized by a T. congolense-specific monoclonal antibody (mAb) Tc6/42.6.4. The identified molecule was expressed as a recombinant protein in E. coli and was tested in several immunoassays for its ability to interact with the mAb. The three dimensional structure of the protein was mo

SUBMITTER: Eyford BA 

PROVIDER: S-EPMC4824491 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature