Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals.

ABSTRACT: Understanding the role of protein turnover in the maintenance of proteostasis requires accurate measurements of the rates of replacement of proteins in complex systems, such as intact animals. Moreover, any investigation of allometric scaling of protein turnover is likely to include species for which fully annotated proteomes are not available. We have used dietary administration of stable isotope labeled lysine to assess protein turnover rates for proteins from four tissues in the bank vole,Myodes glareolus The annotated genome for this species is not available, so protein identification was attained through cross-species matching to the mouse. For proteins for which confident identifications were derived, the pattern of lysine incorporation over 40 days was used to define the rate of syn

SUBMITTER: Hammond DE 

PROVIDER: S-EPMC4824850 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature