Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring.

ABSTRACT: Defining intracellular protein concentration is critical in molecular systems biology. Although strategies for determining relative protein changes are available, defining robust absolute values in copies per cell has proven significantly more challenging. Here we present a reference data set quantifying over 1800Saccharomyces cerevisiaeproteins by direct means using protein-specific stable-isotope labeled internal standards and selected reaction monitoring (SRM) mass spectrometry, far exceeding any previous study. This was achieved by careful design of over 100 QconCAT recombinant proteins as standards, defining 1167 proteins in terms of copies per cell and upper limits on a further 668, with robust CVs routinely less than 20%. The selected reaction monitoring-derived proteome is compared

SUBMITTER: Lawless C 

PROVIDER: S-EPMC4824857 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature