Transcription Elongation Factor NusA Is a General Antagonist of Rho-dependent Termination in Escherichia coli.

ABSTRACT: NusA is an essential protein that binds to RNA polymerase and also to the nascent RNA and influences transcription by inducing pausing and facilitating the process of transcription termination/antitermination. Its participation in Rho-dependent transcription termination has been perceived, but the molecular nature of this involvement is not known. We hypothesized that, because both Rho and NusA are RNA-binding proteins and have the potential to target the same RNA, the latter is likely to influence the global pattern of the Rho-dependent termination. Analyses of the nascent RNA binding properties and consequent effects on the Rho-dependent termination functions of specific NusA-RNA binding domain mutants revealed an existence of Rho-NusA direct competition for the overlappingnut(NusA-bindi

SUBMITTER: Qayyum MZ 

PROVIDER: S-EPMC4825012 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature