Atrazine exposure causes mitochondrial toxicity in liver and muscle cell lines.

ABSTRACT:

Objective

Chronic exposure to atrazine and other pesticides is reported to cause metabolic disorders, yet information on effects of atrazine on expression of genes relevant to mitochondrial function is largely missing. In the present study, therefore, we investigated the expression of a battery of nuclear- and mitochondrial-encoded genes involved in oxidative phosphorylation (OXPHOS) in human liver (HepG2) and rat muscle (L6) cell lines due to short-term atrazine exposure.

Materials and methods

We have determined the EC50 values of atrazine for cytotoxicity and mitochondrial toxicity (mitotoxicity) in terms of adenosine triphosphate (ATP) content in HepG2 and L6 cells. Further, the mRNA expression of nuclear- and mitochondrial-encoded genes was analyzed using quantitative re

SUBMITTER: Sagarkar S 

PROVIDER: S-EPMC4825440 | biostudies-literature | 2016 Mar-Apr

REPOSITORIES: biostudies-literature