Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis.

ABSTRACT: Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each c

SUBMITTER: Cap M 

PROVIDER: S-EPMC4825631 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature