Identification of Polymorphisms Associated with Drought Adaptation QTL in Brassica napus by Resequencing.

ABSTRACT: Brassica napus is a globally important oilseed for which little is known about the genetics of drought adaptation. We previously mapped twelve quantitative trait loci (QTL) underlying drought-related traits in a biparental mapping population created from a cross between winter and spring B. napus cultivars. Here we resequence the genomes of the mapping population parents to identify genetic diversity across the genome and within QTL regions. We sequenced each parental cultivar on the Illumina HiSeq platform to a minimum depth of 23 × and performed a reference based assembly in order to describe the molecular variation differentiating them at the scale of the genome, QTL and gene. Genome-wide patterns of variation were characterized by an overall higher single nucleotide polymorphism (SNP)

SUBMITTER: Fletcher RS 

PROVIDER: S-EPMC4825650 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature