Investigating the effect of drought stress on expression of WRKY1 and EREBP1 genes and antioxidant enzyme activities in lemon balm (Melissa Officinalis L.).

ABSTRACT: Drought stress is a severe environmental constraint to plant productivity. In this study, gene expression of WRKY1 and EREBP1 genes and activity of two enzymes were investigated in two genotypes, G9 (as resistance) and G12 (as susceptible) of 10 days-old lemon balm seedlings. Experiments were done according to factorial design on the base of completely randomized design with three replications for expression analysis and enzyme assays. Seedlings were cultured in MS medium suspensions, including 0, 3, 6, 12 and 15 % w/v PEG 6000. Leaf samples were subsequently collected at 0, 3, 6, 24, 48, 72 h after culture. According to the results of the enzyme assay, SOD activity in resistant genotype was more than in sensitive one. POD activity was high in G9 in severe drought condition and in G12 this

SUBMITTER: Rahimi M 

PROVIDER: S-EPMC4826382 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature