Tobacco smoking is associated with DNA methylation of diabetes susceptibility genes.

ABSTRACT:

Aims/hypothesis

Tobacco smoking, a risk factor for diabetes, is an established modifier of DNA methylation. We hypothesised that tobacco smoking modifies DNA methylation of genes previously identified for diabetes.

Methods

We annotated CpG sites available on the Illumina Human Methylation 450K array to diabetes genes previously identified by genome-wide association studies (GWAS), and investigated them for an association with smoking by comparing current to never smokers. The discovery study consisted of 630 individuals (Bonferroni-corrected p = 1.4 × 10(-5)), and we sought replication in an independent sample of 674 individuals. The replicated sites were tested for association with nearby genetic variants and gene expression and fasting glucose and insulin levels.

Result

SUBMITTER: Ligthart S 

PROVIDER: S-EPMC4826423 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature