Characterization of Interstrand DNA-DNA Cross-Links Using the α-Hemolysin Protein Nanopore.

ABSTRACT: Nanopore-based sensors have been studied extensively as potential tools for DNA sequencing, characterization of epigenetic modifications such as 5-methylcytosine, and detection of microRNA biomarkers. In the studies described here, the α-hemolysin protein nanopore embedded in a lipid bilayer was used for the detection and characterization of interstrand cross-links in duplex DNA. Interstrand cross-links are important lesions in medicinal chemistry and toxicology because they prevent the strand separation that is required for read-out of genetic information from DNA in cells. In addition, interstrand cross-links are used for the stabilization of duplex DNA in structural biology and materials science. Cross-linked DNA fragments produced unmistakable current signatures in the nanopore experim

SUBMITTER: Zhang X 

PROVIDER: S-EPMC4826734 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature