Bright Fluorescent Nanotags from Bottlebrush Polymers with DNA-Tipped Bristles.

ABSTRACT: Bright signal outputs are needed for fluorescence detection of biomolecules at their native expression levels. Increasing the number of labels on a probe often results in crowding-induced self-quenching of chromophores, and maintaining the function of the targeting moiety (e.g., an antibody) is a concern. Here we demonstrate a simple method to accommodate thousands of fluorescent dye molecules on a single antibody probe while avoiding the negative effects of self-quenching. We use a bottlebrush polymer from which extend hundreds of duplex DNA strands that can accommodate hundreds of covalently attached and/or thousands of noncovalently intercalated fluorescent dyes. This polymer-DNA assembly sequesters the intercalated fluorophores against dissociation and can be tethered through DNA hybri

SUBMITTER: Fouz MF 

PROVIDER: S-EPMC4827471 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature