Conformational evaluation of HIV-1 trimeric envelope glycoproteins using a cell-based ELISA assay.

ABSTRACT: HIV-1 envelope glycoproteins (Env) mediate viral entry into target cells and are essential to the infectious cycle. Understanding how those glycoproteins are able to fuel the fusion process through their conformational changes could lead to the design of better, more effective immunogens for vaccine strategies. Here we describe a cell-based ELISA assay that allows studying the recognition of trimeric HIV-1 Env by monoclonal antibodies. Following expression of HIV-1 trimeric Env at the surface of transfected cells, conformation specific anti-Env antibodies are incubated with the cells. A horseradish peroxidase-conjugated secondary antibody and a simple chemiluminescence reaction are then used to detect bound antibodies. This system is highly flexible and can detect Env conformational change

SUBMITTER: Veillette M 

PROVIDER: S-EPMC4828074 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature