Accurate and Phenol Free DNA Sexing of Day 30 Porcine Embryos by PCR.

ABSTRACT: Research into prenatal programming in the pig has shown that the sex of the developing embryo or fetus can influence the developmental outcome. Therefore, the ability to determine an embryo's sex is necessary in many experiments particularly regarding early development. The present protocol demonstrates an inexpensive, rapid and non-toxic preparation of pig genomic DNA for use with PCR. Day 30 embryos must be humanely collected according to the guidelines established by Institutional Animal Policy and Welfare Committees for the present protocol. The preparation of the whole embryo for this PCR based sexing technique simply involves grinding the frozen embryo to a fine powder using a pre-chilled mortar and pestle. PCR-quality DNA is released from a small amount of embryo powder by applying

SUBMITTER: Blanes MS 

PROVIDER: S-EPMC4828158 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature