Quantitation of Vacuolar Sugar Transporter Abundance Changes Using QconCAT Synthtetic Peptides.

ABSTRACT: Measurements of protein abundance changes are important for biological conclusions on protein-related processes such as activity or complex formation. Proteomic analyses in general are almost routine tasks in many laboratories, but a precise and quantitative description of (absolute) protein abundance changes require careful experimental design and precise data quality. Today, a vast choice of metabolic labeling and label-free quantitation protocols are available, but the trade-off between quantitative precision and proteome coverage of quantified proteins including missing value problems remain. Here, we provide an example of a targeted proteomic approach using artificial standard proteins consisting of concatenated peptides of interest (QconCAT) to specifically quantify abiotic stress-in

SUBMITTER: Pertl-Obermeyer H 

PROVIDER: S-EPMC4828444 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature