Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes.

ABSTRACT: Cell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value. Herein, we developed an automated micropipette-based quantitative microinjection technology that can deliver precise amounts of materials into cells. The developed microinjection system achieved precise single-cell microinjection by pre-patterning cells in an array and controlling the amount of substance delivered based on injection pressure and time. The precision of the proposed injection technique was examined by comparing the fluorescence intensities of fluorescent dye droplets with a standard concentration and water droplets with a known injection amoun

SUBMITTER: Chow YT 

PROVIDER: S-EPMC4828701 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature