A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis.

ABSTRACT: Whole mount in situ hybridization (WMISH) is a technique that allows for the spatial resolution of nucleic acid molecules (often mRNAs) within a 'whole mount' tissue preparation, or developmental stage (such as an embryo or larva) of interest. WMISH is extremely powerful because it can significantly contribute to the functional characterization of complex metazoan genomes, a challenge that is becoming more of a bottleneck with the deluge of next generation sequence data. Despite the conceptual simplicity of the technique much time is often needed to optimize the various parameters inherent to WMISH experiments for novel model systems; subtle differences in the cellular and biochemical properties between tissue types and developmental stages mean that a single WMISH method may not be approp

SUBMITTER: Jackson DJ 

PROVIDER: S-EPMC4829025 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature