ADAM17 Inhibitors Attenuate Corneal Epithelial Detachment Induced by Mustard Exposure.

ABSTRACT:

Purpose

Sulfur mustard, nitrogen mustard (NM), and 2-chloroethyl ethyl sulfide all cause corneal injury with epithelial-stromal separation, differing only by degree. Injury can resolve in a few weeks or develop into chronic corneal problems. These vesicants induce microbullae at the epithelial-stromal junction, which is partially caused by cleavage of transmembranous hemidesmosomal collagen XVII, a component anchoring the epithelium to the stroma. ADAM17 is an enzyme involved in wound healing and is able to cleave collagen XVII. The activity of ADAM17 was inhibited in vesicant-exposed corneas by four different hydroxamates, to evaluate their therapeutic potential when applied 2 hours after exposure, thereby allowing ADAM17 to perform its early steps in wound healing.

Methods

SUBMITTER: DeSantis-Rodrigues A 

PROVIDER: S-EPMC4829087 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature