Purification and characterization of endo β-1,4-D-glucanase from Trichoderma harzianum strain HZN11 and its application in production of bioethanol from sweet sorghum bagasse.

ABSTRACT: An acidophilic-solvent-thermostable endo β-1,4-D-glucanase produced from a potential Trichoderma harzianum strain HZN11 was purified to homogeneity by DEAE-Sepharose and Sephadex G-100 chromatography with 33.12 fold purification with specific activity of 66.25 U/mg and molecular mass of ~55 kDa. The optimum temperature and pH were 60 °C and 5.5 retaining 76 and 85 % of activity after 3 h, respectively. It showed stability between pH 4.5-6.0 and temperature between 50-70 °C indicating thermostability. Endo β-1,4-D-glucanase was activated by Ca²⁺ and Mg²⁺ but inhibited by Hg²⁺, Pb²⁺ and Cd²⁺. The effect of thiol reagents, metal chelators, oxidizing agents and surfactants on enzyme activity has been studied. Purified endo β-1,4-D-glucanas

SUBMITTER: Bagewadi ZK 

PROVIDER: S-EPMC4829572 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature