MLVA and MLST typing of Brucella from Qinghai, China.

ABSTRACT:

Background

The Qinghai-Tibet Plateau (QTP) of China is an extensive pastoral and semi-pastoral area, and because of poverty and bad hygiene conditions, Brucella is highly prevalent in this region. In order to adequately prevent this disease in the QTP region it is important to determine the identity of Brucella species that caused the infection.

Methods

A total of 65 Brucella isolates were obtained from human, livestock and wild animals in Qinghai, a Chinese province in east of the QTP. Two molecular typing methods, MLVA (multi-locus variable-number tandem-repeat analysis) and MLST (multi locus sequence typing) were used to identify the species and genotypes of these isolates.

Findings

Both MLVA and MLST typing methods classified the 65 isolates into three species, B.

SUBMITTER: Ma JY 

PROVIDER: S-EPMC4830052 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature