Analysis of the microbiome: Advantages of whole genome shotgun versus 16S amplicon sequencing.

ABSTRACT: The human microbiome has emerged as a major player in regulating human health and disease. Translational studies of the microbiome have the potential to indicate clinical applications such as fecal transplants and probiotics. However, one major issue is accurate identification of microbes constituting the microbiota. Studies of the microbiome have frequently utilized sequencing of the conserved 16S ribosomal RNA (rRNA) gene. We present a comparative study of an alternative approach using whole genome shotgun sequencing (WGS). In the present study, we analyzed the human fecal microbiome compiling a total of 194.1 × 10(6) reads from a single sample using multiple sequencing methods and platforms. Specifically, after establishing the reproducibility of our methods with extensive multiplexing,

SUBMITTER: Ranjan R 

PROVIDER: S-EPMC4830092 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature