KPC enzymes in the UK: an analysis of the first 160 cases outside the North-West region.

ABSTRACT:

Objectives

Klebsiella pneumoniae carbapenemases (KPCs) have been increasingly reported in the UK since 2003. We analysed patient and isolate data for KPC-positive bacteria confirmed by the national reference laboratory from UK laboratories from August 2003 to August 2014, excluding North-West England, where the epidemiology has previously been studied.

Methods

MICs were determined by BSAC agar dilution. Carbapenem-resistant isolates lacking imipenem/EDTA synergy were tested by PCR for blaKPC. MLST and blaKPC sequencing were performed on a subset of isolates. Plasmid analysis was performed by transformation, PCR-based replicon typing and, in some cases, whole-plasmid sequencing. Patient data provided by the sending laboratories were reviewed.

Results

Two hundred and te

SUBMITTER: Findlay J 

PROVIDER: S-EPMC4830413 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature