Transcriptomic profiling of Methylococcus capsulatus (Bath) during growth with two different methane monooxygenases.

ABSTRACT: Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). The expression of these two MMO's is tightly controlled by the availability of copper in the growth medium, but the underlying mechanisms and the number of genes involved in this switch in methane oxidation is not yet fully elucidated. Microarray analyses were used to assess the transcriptome in cells producing either pMMO or sMMO. A total of 137 genes were differentially expressed, with 87 genes showing a significant up-regulation during sMMO production. The majority of the differentially expressed genes could be assigned to functional roles in the energy metabolism and transport. Furthermore, three copper responding gene clusters were discovered, in

SUBMITTER: Larsen O 

PROVIDER: S-EPMC4831470 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature