Lineage Tracking for Probing Heritable Phenotypes at Single-Cell Resolution.

ABSTRACT: Determining the phenotype and genotype of single cells is central to understand microbial evolution. DNA sequencing technologies allow the detection of mutants at high resolution, but similar approaches for phenotypic analyses are still lacking. We show that a drop-based millifluidic system enables the detection of heritable phenotypic changes in evolving bacterial populations. At time intervals, cells were sampled and individually compartmentalized in 100 nL drops. Growth through 15 generations was monitored using a fluorescent protein reporter. Amplification of heritable changes-via growth-over multiple generations yields phenotypically distinct clusters reflecting variation relevant for evolution. To demonstrate the utility of this approach, we follow the evolution of Escherichia coli p

SUBMITTER: Cottinet D 

PROVIDER: S-EPMC4831777 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature