Advancing Edge Speeds of Epithelial Monolayers Depend on Their Initial Confining Geometry.

ABSTRACT: Collective cell migrations are essential in several physiological processes and are driven by both chemical and mechanical cues. The roles of substrate stiffness and confinement on collective migrations have been investigated in recent years, however few studies have addressed how geometric shapes influence collective cell migrations. Here, we address the hypothesis that the relative position of a cell within the confinement influences its motility. Monolayers of two types of epithelial cells--MCF7, a breast epithelial cancer cell line, and MDCK, a control epithelial cell line--were confined within circular, square, and cross-shaped stencils and their migration velocities were quantified upon release of the constraint using particle image velocimetry. The choice of stencil geometry allowed

SUBMITTER: Kollimada SA 

PROVIDER: S-EPMC4831833 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature