Proteomic Characterization of Middle Ear Fluid Confirms Neutrophil Extracellular Traps as a Predominant Innate Immune Response in Chronic Otitis Media.

ABSTRACT:

Background

Chronic Otitis Media (COM) is characterized by middle ear effusion (MEE) and conductive hearing loss. MEE reflect mucus hypersecretion, but global proteomic profiling of the mucosal components are limited.

Objective

This study aimed at characterizing the proteome of MEEs from children with COM with the goal of elucidating important innate immune responses.

Method

MEEs were collected from children (n = 49) with COM undergoing myringotomy. Mass spectrometry was employed for proteomic profiling in nine samples. Independent samples were further analyzed by cytokine multiplex assay, immunoblotting, neutrophil elastase activity, next generation DNA sequencing, and/or immunofluorescence analysis.

Results

109 unique and common proteins were identified by MS.

SUBMITTER: Val S 

PROVIDER: S-EPMC4831838 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature