A prefoldin-associated WD-repeat protein (WDR92) is required for the correct architectural assembly of motile cilia.

ABSTRACT: WDR92 is a highly conserved WD-repeat protein that has been proposed to be involved in apoptosis and also to be part of a prefoldin-like cochaperone complex. We found that WDR92 has a phylogenetic signature that is generally compatible with it playing a role in the assembly or function of specifically motile cilia. To test this hypothesis, we performed an RNAi-based knockdown of WDR92 gene expression in the planarianSchmidtea mediterraneaand were able to achieve a robust reduction in mRNA expression to levels undetectable under our standard RT-PCR conditions. We found that this treatment resulted in a dramatic reduction in the rate of organismal movement that was caused by a switch in the mode of locomotion from smooth, cilia-driven gliding to muscle-based, peristaltic contractions. Althou

SUBMITTER: Patel-King RS 

PROVIDER: S-EPMC4831875 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature