Application of an RNA amplification method for reliable single-cell transcriptome analysis.

ABSTRACT: Diverse cell types have unique transcriptional signatures that are best interrogated at single-cell resolution. Here we describe a novel RNA amplification approach that allows for high fidelity gene profiling of individual cells. This technique significantly diminishes the problem of 3' bias, enabling detection of all regions of transcripts, including the recognition of mRNA with short or completely absent poly(A) tails, identification of noncoding RNAs, and discovery of the full array of splice isoforms from any given gene product. We assess this technique using statistical and bioinformatics analyses of microarray data to establish the limitations of the method. To demonstrate applicability, we profiled individual cells isolated from the mouse subventricular zone (SVZ)-a well-characteriz

SUBMITTER: Suslov O 

PROVIDER: S-EPMC4832400 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature