The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing.

ABSTRACT:

Background

The cohesin complex consists of multiple core subunits that play critical roles in mitosis and transcriptional regulation. The cohesin-associated protein Wapal plays a central role in off-loading cohesin to facilitate sister chromatid separation, but its role in regulating mammalian gene expression is not understood. We used embryonic stem cells as a model, given that the well-defined transcriptional regulatory circuits were established through master transcription factors and epigenetic pathways that regulate their ability to maintain a pluripotent state.

Results

RNAi-mediated depletion of Wapal causes a loss of pluripotency, phenocopying loss of core cohesin subunits. Using chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq), we dete

SUBMITTER: Stelloh C 

PROVIDER: S-EPMC4832553 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature