Mitochondrial and inflammatory changes in sporadic inclusion body myositis.

ABSTRACT:

Aims

Sporadic inclusion body myositis (sIBM) is the most common late onset muscle disease causing progressive weakness. In light of the lack of effective treatment, we investigated potential causes underlying muscle wasting. We hypothesized that accumulation of mitochondrial respiratory deficiency in muscle fibres may lead to fibre atrophy and degeneration, contributing to muscle mass reduction.

Methods

Histochemical and immunohistochemical analyses were performed on muscle biopsies from 16 sIBM patients to detect activity of mitochondrial enzymes and expression of mitochondrial respiratory chain proteins along with inflammatory markers respectively. Mitochondrial DNA mutations were assessed in single muscle fibres using real-time PCR.

Results

We identified respirator

SUBMITTER: Rygiel KA 

PROVIDER: S-EPMC4833191 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature