Expression of granulocyte colony stimulating factor (GCSF) in Hansenula polymorpha.

ABSTRACT:

Background and objectives

During past decades Hansenula polymorpha has attracted global attention for the expression of recombinant proteins due to its high growth rate, minimal nutritional porequirements and use of methanol as a low cost inducer.

Materials and methods

The corresponding nucleotide sequences for the expression of heterologous genes in Hansenula poylmorpha were extracted and assembled in an E. coli vector. The constructed expression cassette included formate dehydrogenase promoter (pFMD), a secretory signal sequence, a multiple cloning site (MCS) and methanol oxidase (MOX) terminator. Zeocin resistance gene fragment and complete cDNA encoding granulocyte colony stimulating factor (GCSF) were cloned downstream of the expression cassette in-frame with signal seq

SUBMITTER: Talebkhan Y 

PROVIDER: S-EPMC4833737 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature