The Effect of Basepair Mismatch on DNA Strand Displacement.

ABSTRACT: DNA strand displacement is a key reaction in DNA homologous recombination and DNA mismatch repair and is also heavily utilized in DNA-based computation and locomotion. Despite its ubiquity in science and engineering, sequence-dependent effects of displacement kinetics have not been extensively characterized. Here, we measured toehold-mediated strand displacement kinetics using single-molecule fluorescence in the presence of a single basepair mismatch. The apparent displacement rate varied significantly when the mismatch was introduced in the invading DNA strand. The rate generally decreased as the mismatch in the invader was encountered earlier in displacement. Our data indicate that a single base pair mismatch in the invader stalls branch migration and displacement occurs via direct disso

SUBMITTER: Broadwater DWB 

PROVIDER: S-EPMC4833772 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature