Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis.

ABSTRACT: The plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis,

SUBMITTER: Volkner M 

PROVIDER: S-EPMC4834051 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature