Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection.

ABSTRACT: A complete understanding of host-parasite interactions must necessarily include the identification and characterization of gene products expressed by both parties during the infectious process. We have developed a new screen to identify bacterial genes that are transcriptionally induced during infection of a host animal. The method is based on pre-selection of strains carrying tnpR operon fusions (encoding resolvase, a site-specific DNA recombinase) which are not expressed in vitro, followed by screening for a subset of these strains that subsequently express resolvase within the host environment. The latter subset was recognized as recombinants that had deleted a resolvase-specific reporter construct. Thirteen transcription units of Vibrio cholerae were identified that were induced during

SUBMITTER: Camilli A 

PROVIDER: S-EPMC4834211 | biostudies-literature | 1995 Nov

REPOSITORIES: biostudies-literature